donor stock number: JMA619
Resource Type: plasmid
Availability: available
Donors:- Jose Alonso
Donation Date: 08/28/2019
Date Released: 10/18/2019
Description:
AraYpet-FRT-TetA-FRT recombineering cassette. To generate this cassette, the tetracycline resistance gene, TetR, and corresponding promoter sequences were amplified from the genomic DNA of E. coli recombineering strain SW102, and the PCR product was then employed in a recombineering reaction to insert the FRT-Tet-FRT sequences in the Universal AraYpet cassette.
Growth Requirement: grow in LB media at 32C for 48 hours
Marker: kanamycin
Background:
ABRC Comment:
Format Shipped: bacterial stab
Base / Commercial Price: $15 / $120
Plasmid
Promoter:
Reporter:
Type: cloning vector
E. coli SW105
Additional Information
Quality Control Comments
There is no quality control data for this stock.