donor stock number: pDamBOB_Cloning
Resource Type: plasmid
Availability: available
Donors:- Matthew Brooks
- Gloria Coruzzi
- Jose Alvarez
Donation Date: 01/17/2023
Date Released: 02/07/2023
Description:
Plasmid for transient DamID (DNA adenine methyltransferase identification) in Arabidopsis protoplasts. Used for Bsa1 restriction enzyme mediated cloning to insert transcription factor of interest downstream of the Dam-GR fusion. Contains a filler mutated bZIP1 sequence that will drop out after Bsa1 digestion. Must be maintained in PIR1 cells.
Growth Requirement: grow in LB media at 37C overnight
Marker: ampicillin
Background:
ABRC Comment:
Format Shipped: bacterial stab
Base / Commercial Price: $15 / $120
Plasmid
Promoter:
Reporter: mCherry
Type: cloning vector
E. coli PIR1
Additional Information
| DOI | PubMed |
|---|---|
| 10.1038/s41467-020-14979-6 | 32123177 |
Quality Control Comments
There is no quality control data for this stock.