NASC stock number: N2110796
donor stock number: dex control x PI4P marker
other name: MAP-3xmCH x mCit-P4M
Resource Type: seed
Availability: available
Donors:- Yvon Jaillais
Donation Date: 09/13/2021
Date Released: 10/05/2021
Description:
Transgenic line generated from a cross between the iDePP dex control line (MAP-3xmCH) (CS2110787) and a biosensor for PI4P (mCIT-P4M(SidM)) (CS2107346). Control that reveals that removal of PI(4,5)P2 from the plasma membrane by iDePP is not a side effect from dexamethasone treatment and is specific to PI(4,5)P2, not PI4P. Col-0 background. Hygromycin and BASTA resistant. Homozygous for the transgenes. This line is part of the iDePP set of lines (CS2110797).
Growth Requirement: induction upon dexamathasone (dex) treatment
Marker: BASTA, hygromycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
mCITRINE (cYFP) expression localized to the plasma membrane
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1038/s41477-021-00907-z | 34007035 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pUBQ10::mCITRINE-P4M(SidM) | mCITRINE (cYFP) fluorescent protein fused to a carboxy (C)-terminal P4M domain of the SidM effector protein from Legionella pneumophila | UBQ10 | mCITRINE | BASTA |
| UBQ10prom::GVG::6UAS::MAP-3xmCHERRY | 3xmCherry fused to a membrane anchoring peptide, expression driven by a dexamethasone (dex)-inducible promoter | UBQ10 | 3xmCherry | hygromycin |
Quality Control Comments
There is no quality control data for this stock.