NASC stock number: N29912
donor stock number: 3bhsd-2
Resource Type: seed
Availability: available
Donors:- Georg Jander
Donation Date: 04/29/2024
Date Released: 05/09/2024
Description:
CRISPR/Cas9 generated knockout of EcCYP87A126, the cytochrome P450 responsible for sterol side chain cleavage and the initiation of cardenolide biosynthesis in Erysimum cheiranthoides. One bp insertion in coding sequence of CYP87A126 (Erche04g002680) results in loss of function and complete loss of cardenolide accumulation in plant tissues. No obvious visual phenotype or special growth requirements.
Growth Requirement: Stratification for 2 days at 4C recommended
Marker:
Background: Elbtalaue
ABRC Comment:
Format Shipped: 15 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
5beta-cardenolide synthesis reduced, accumulation of delta5-cardenolides
Erysimum cheiranthoides 330168
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| SpCas9 | SpCas9 with gRNA targeting Erche07g001535. | AtRPS5A | DsRed under CaMV promoter |
Quality Control Comments
There is no quality control data for this stock.