Name / Stock Number: CS29915

NASC stock number: N29915

donor stock number: 3bhsd/3ksi

Resource Type: seed

Availability: available

Donors:
  • Georg Jander

Donation Date: 04/29/2024

Date Released: 05/09/2024

Description:

CRISPR/Cas9 generated knockout of EcCYP716A418, a cytochrome P450 that appears to be involved in cardenolide hydroxylation, though enzyme activity has not been confirmed. One bp insertion in coding sequence of CYP716A418 (Erche02g006810) results in hyperaccumulation of digitoxigenin glycosides and a lack of more hydroxylated cardenolides such as cannogenol glycosides and strophanthidin glycosides. No obvious visual phenotype or special growth requirements.

Growth Requirement: Stratification for 2 days at 4C recommended

Marker:

Background: Elbtalaue

ABRC Comment:

Format Shipped: 15 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

2

5beta-cardenolide synthesis reduced, accumulation of delta5-cardenolides

Erysimum cheiranthoides 330168

Additional Information

DOI PubMed
10.1101/2024.04.10.588904 38645095

Quality Control Comments

There is no quality control data for this stock.