NASC stock number: N69828
donor stock number: eml1-2 pEML1::GFP-EML1
Resource Type: seed
Availability: available
Donors:- Jelena Brkljacic
- Erich Grotewold
Donation Date: 05/16/2017
Date Released: 05/29/2019
Description:
pEML1::GFP-EML1 minigene was constructed using Multisite Gateway Technology (Invitrogen) by fusing the promoter region of EML1 (1.35kb upstream of the EML1 coding sequence) to the sGFP sequence in frame with the EML1 coding sequence into pH7m34GW. pEML1::GFP-EML1 was transformed into eml1-2 mutant plants (CS69823) by floral dip. Hygromycin resistant. Homozygous for the transgene.
Growth Requirement: none
Marker: hygromycin
Background: Ler (Landsberg erecta)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
No visible phenotype.
Arabidopsis thaliana 3702
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| GTDs | gene trap transposon | GUS | kanamycin | |
| pEML1::GFP-EML1 | minigene constructed using Multisite Gateway Technology (Invitrogen) by fusing the promoter region of EML1 (1.1kb upstream of the EML1 coding sequence) to the sGFP sequence in frame with the EML1 coding sequence | 1.35kb upstream of the EML1 coding sequence | GFP | hygromycin |
Quality Control Comments
There is no quality control data for this stock.