donor stock number: 5:25
NASC stock number: N71721
Resource Type: seed
Availability: available
Donors:- Antonius Matzke
- Marjori Matzke
Donation Date: 11/26/2018
Date Released: 12/19/2018
Description:
Chromosome 4 fluorescence-tagged tetraploid transgenic line expressing construct 5, an enhanced YFP and tet repressor/operator system, and a second construct containing the RPS5 promoter to drive expression of genes encoding the Tet repressor-EYFP fusion protein (TY) and histone H2B fused to monomeric DsRed (modification of construct C5; Figure 1, Matzke et al., 2010). Col-0 plants were transformed by the floral dip method. Kanamycin and phosphinotricin resistant. Homozygous for the transgenes. Tetraploid plants were produced by colchicine treatment.
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
4
No visible phenotype
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1073/pnas.1807796115 | 30429332 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| Construct 5 | construct containing an enhanced YFP and tet repressor/operator system | CaMV 35S | EYFP | kanamycin |
Quality Control Comments
There is no quality control data for this stock.