donor stock number: de48
NASC stock number: N71746
other name: cdka;1
other name: ProCDKA;1::CDKA;1-T14D;Y15E
Resource Type: seed
Availability: available
Donors:- Nico Dissmeyer
Donation Date: 12/17/2018
Date Released: 01/22/2019
Description:
Transgenic line expressing CDKA;1 phosphomimicry substitution variant DE (T14D;Y15E) under the control of the CDKA;1 promoter in a heterozygous cdka;1-1 mutant background. Basta resistant. Homozygous for the transgene.
Growth Requirement: maintain as heterozygote, cdc2a-1 mutants are sterile
Marker: BASTA
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Cell cycle hypomorph. Both wild-type and mutant, single sperm pollen can be found. Plants homozygous for cdka;1 are sterile.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1105/tpc.109.070417 | 19948791 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pROK2 | T-DNA | kanamycin | ||
| ProCDKA;1::CDKA;1-T14D;Y15E | CDKA;1 phosphomimicry substitution variant DE (T14D;Y15E) under the control of the CDKA;1 promoter | CDKA;1 | BASTA |
Quality Control Comments
There is no quality control data for this stock.