donor stock number: 35S:YFP:PIF3-507 in pif3-3
NASC stock number: N71749
Resource Type: seed
Availability: available
Donors:- Jutta Dalton
- Peter Quail
Donation Date: 12/19/2018
Date Released: 01/22/2019
Description:
Transgenic line expressing a YFP:PIF3-507 protein fusion under the control of the CaMV 35S promoter in a homozygous pif3-3 mutant background. PIF3 cDNA was amplified with primers NLS-F and PIF3-507AA-R using NLS:PIF3 as template. The product was cloned into vector pENTR/D-TOPO and transferred into Gateway binary vector pGWB42 containing a 35S promoter and YFP fusion at the N terminus. pif3-3 plants were transformed by the floral dip method and T1 seeds were selected on both kanamycin- and hygromycin-containing media. Homozygous for the transgene.
Growth Requirement: none
Marker: kanamycin, hygromycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Elongated hypocotyl in continuous R light.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1105/tpc.113.112342 | 23903316 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| 35S:YFP:PIF3-507 | CaMV 35S | YFP | kanamycin, hygromycin |
Quality Control Comments
There is no quality control data for this stock.