NASC stock number: N71750
donor stock number: 35S:GNC-L1
Resource Type: seed
Availability: available
Donors:- Yan Zubo
- G. Schaller
Donation Date: 01/14/2019
Date Released: 02/22/2019
Description:
Transgenic line overexpressing GNC. The genomic sequence of GNC was amplified from Col-0 genomic DNA and cloned into pCR8/GW/TOPO to generate an entry clone. The GNC entry clone was recombined into pEarleyGate 103 for C-terminal fusion with GFP and expression driven by the CaMV 35S promoter. Col-0 plants were transformed by the floral dip method and selection was performed based on resistance to Basta. Homozygous for the transgene.
Growth Requirement: none
Marker: BASTA
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Dark-green leaves, reduced shoot growth, small rosette, slight reduction in rosette leaf number. Enhanced chloroplast production in the roots and in both epidermal pavement and stomatal guard cells. Delayed bolting. Increased NPQ (nonphotochemical energy quenching) and a decrease in the flux of electrons through PSII. Increased stomatal and meristemoid indexes.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1104/pp.112.198705 | 22811435 |
| 10.1104/pp.18.00414 | 30002259 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| 35S:GNC-GFP | genomic sequence of GNC driven by the CaMV 35S promoter, C-terminal fusion with GFP | CaMV 35S | GFP | BASTA |
Quality Control Comments
There is no quality control data for this stock.