NASC stock number: N71757
donor stock number: 35S:GNC-L1 (glk1 glk2)
Resource Type: seed
Availability: available
Donors:- Yan Zubo
- G. Schaller
Donation Date: 01/14/2019
Date Released: 02/22/2019
Description:
Transgenic line overexpressing GNC in the glk1 glk2 double mutant background. The genomic sequence of GNC was amplified from Col-0 genomic DNA and cloned into pCR8/GW/TOPO to generate an entry clone. The GNC entry clone was recombined into the pGWB20 destination binary vector for C-terminal fusion with a 10x Myc tag and expression driven by the CaMV 35S promoter. glk1 glk2 double mutant plants (generated by genetic crossing of CS9805 to CS9806) were transformed by the floral dip method and selection was performed based on resistance to kanamycin. Homozygous for the transgene.
Growth Requirement: none
Marker: kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Partial rescue of the glk1 glk2 mutant phenotype. Small rosette, light-green leaves. Reduced sizes of mesophyll chloroplasts. Enhanced chloroplast production in both epidermal pavement and stomata guard cells, as well as in roots and hypocotyls. Increase in NPQ (nonphotochemical energy quenching) and a decrease in the flux of electrons through PSII.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1104/pp.18.00414 | 30002259 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| dSpm | defective Spm (dSpm) transposon | kanamycin | ||
| 35S:GNC-MYC | genomic sequence of GNC driven by the CaMV 35S promoter, C-terminal fusion with a 10x Myc tag | CaMV 35S | 10x Myc | kanamycin |
Quality Control Comments
There is no quality control data for this stock.