NASC stock number: N71803
other name: tETR1-G2 #1 (etr1-9;ers1-3)
donor stock number: tETR1-G2 #1
Resource Type: seed
Availability: available
Donors:- G. Schaller
Donation Date: 02/18/2019
Date Released: 03/26/2019
Description:
Transgenic expression of a kinase-inactive mutant of ETR1 (tETR1-G2) in the etr1-9;ers1-3 double mutant background. An ETR1 genomic clone containing the promoter and full-length coding regions was subcloned into pALTERII and the Altered Sites Mutagenesis System was used for site-directed mutation of the G2-box of ETR1. This mutation codes for a conversion of Gly-545 (GGG) to Ala (GCG) and a conversion of Gly-547 (GGC) to Ala (GCC). The mutant product was then cloned into binary vector pCAMBIA2380. Since the etr1-9;ers1-3 homozygous double mutant is sterile, the construct was initially transformed into etr1-9/etr1-9;ers1-3/ERS1 plants, and plants homozygous for the mutations and the transgene were identified in subsequent generations. Kanamycin resistant.
Growth Requirement: none
Marker: kanamycin
Background: Ws (Wassilewskija)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Ethylene hyposensitive.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1104/pp.112.196790 | 22467798 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pSKI15 | activation tag T-DNA | 4x CaMV 35S | BASTA | |
| tETR1-G2 | kinase-inactive mutant of ETR1 (tETR1-G2) generated from ETR1 genomic clone containing the promoter and full-length coding regions modified by site-directed mutatagenesis of the G2-box, the mutation codes for a conversion of Gly-545 (GGG) to Ala (GCG) and a conversion of Gly-547 (GGC) to Ala (GCC) | ETR1 | kanamycin |
Quality Control Comments
There is no quality control data for this stock.