NASC stock number: N71815
other name: EIN4-TAP (ein4-4)
donor stock number: EIN4-TAP
Resource Type: seed
Availability: available
Donors:- G. Schaller
Donation Date: 02/18/2019
Date Released: 03/26/2019
Description:
ein4-4 mutant expressing EIN4 driven by its native promoter with C-terminal streptaviden-binding peptide (SBP) and tandem affinity purification (TAP) tags. The region encoding EIN4 along with the upstream promoter sequence was amplified from the Arabidopsis BAC clone F7O18 (GenBank accession no. AC011437) and the PCR product was cloned into vector pCAMBIA1380-SBP-TAP. The construct was transformed into the corresponding single loss-of-function mutant background, ein4-4. Hygromycin B resistant. Homozygous for the transgene.
Growth Requirement: none
Marker: hygromycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
No visible phenotype.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1074/jbc.M800641200 | 18577522 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| EIN4-TAP | EIN4 driven by its native promoter with C-terminal streptaviden-binding peptide (SBP) and tandem affinity purification (TAP) tags | EIN4 | hygromycin |
Quality Control Comments
There is no quality control data for this stock.