NASC stock number: N72197
donor stock number: pRIN4::T7-gRIN4T166A RPM1-myc rpm1-3 rps2-101c rin4
Resource Type: seed
Availability: available
Donors:- Jeff Dangl
Donation Date: 05/22/2019
Date Released: 07/09/2019
Description:
RPM1-myc rpm1-3 rps2-101c rin4 (CS68783) expressing a T7-tagged T166A AvrB binding site (BBS) mutant of RIN4 driven by the native RIN4 promoter. The T166A BBS mutant of RIN4 was generated by site-directed mutagenesis. The transgene was cloned into the pBAR1-GW destination vector for Basta selection and plant transformation was performed using the floral dip method. Homozygous for the transgenes.
Growth Requirement: none
Marker: BASTA
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Full loss-of-function for RPM1 against P. syringae type III effector protein AvrB, and partial loss-of-function for RPM1 against P. syringae type II effector protein AvrRpm1.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1016/j.chom.2011.01.009 | 21320695 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pROK2 | T-DNA | kanamycin | ||
| pRPM1::RPM1::myc | ||||
| pRIN4::T7-gRIN4T166A | T7-tagged nonphosphorylable T166A mutant of RIN4 driven by the native RIN4 promoter | RIN4 | BASTA |
Quality Control Comments
There is no quality control data for this stock.