NASC stock number: N72458
donor stock number: 35S::AGB1 W109A line 4-4C
Resource Type: seed
Availability: available
Donors:- Alan Jones
Donation Date: 02/01/2021
Date Released: 03/12/2021
Description:
Transgenic line stably expressing AGB1 variant in the Gbeta-null agb1-2 mutant background. The binary construct in the pGWB backbone was introduced into Agrobacterium tumefaciens by electroporation and then into the agb1-2 mutant (CS6536) by floral dipping. Hygromycin resistant. Homozygous for the transgene.
Growth Requirement: none
Marker: hygromycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Partial complementation of the agb1-2 hypocotyl, lateral root, and ABA-hypersensitivity phenotypes. Complete rescue of agb1-2 scorable leaf, silique, fungal pathogen hypersusceptibility, and flg22-triggered ROS formation phenotypes.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1104/pp.112.196337 | 22570469 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| 35S::AGB1 W109A | AGB1 variant generated through site-directed mutagenesis expressed under the control of the 35S promoter | CaMV 35S | hygromycin |
Quality Control Comments
There is no quality control data for this stock.