NASC stock number: N72493
donor stock number: B16P1::PIF1-4xmyc
Resource Type: seed
Availability: available
Donors:- Peter Quail
- Jutta Dalton
Donation Date: 02/10/2021
Date Released: 05/20/2021
Description:
B16P1::PIF1-4xmyc transgenic line. The full legth PIF1 sequence without stop codon and the 3 kb upstream of the ATG start codon were amplified via PCR from genomic DNA and cloned into a Gateway entry vector. LR reaction into binary vector pGWB16 was performed and Col plants were transformed via floral dip. Kanamycin and hygromycin resistant. Homozygous for the transgene.
Growth Requirement: none
Marker: kanamycin, hygromycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| B16P1::PIF1-4xmyc | PIF1 fused to a 4xmyc tag | B16P1 | 4xmyc | kanamycin, hygromycin |
Quality Control Comments
There is no quality control data for this stock.