NASC stock number: N72513
donor stock number: HSP101-PP7-3
Resource Type: seed
Availability: not available
Donors:- Krishna Niyogi
Donation Date: 04/06/2021
Date Released: 06/02/2021
Description:
Transgenic line. Col-0 plants were transformed with the UPG plasmid (Addgene #161003) coding for a PCP-mGFP5 fusion driven by the Arabidopsis UBQ10 constitutive promoter and a second construct containing a PP7 reporter driven by the Arabidopsis HSP101 promoter and a H2B-mScarlet nuclear marker (based on Addgene plasmid #161006). Hygromycin and kanamycin resistant. Potentially segregating for the transgenes.
Growth Requirement: none
Marker: kanamycin, hygromycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
GFP fluorescence in the nucleus and the cytoplasm of all cells. Nuclear fluorescent punctae in response to protein misfolding stress (e.g., heat shock).
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1101/2020.08.30.274621 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| AL13Rb-HSP101 | PCP-mGFP fusion and a PP7 reporter driven by the HSP101 promoter | UBQ10; HSP101 | GFP; PP7 | kanamycin, hygromycin |
Quality Control Comments
There is no quality control data for this stock.