NASC stock number: N72563
donor stock number: AGO4::FLAG-AGO4(D742A) T5-19
other name: ago4-4/6-2/9-1/D742A
Resource Type: seed
Availability: available
Donors:- Michael Axtell
Donation Date: 07/09/2021
Date Released: 08/11/2021
Description:
Slicing-defective AGO4 driven by the native AGO4 promoter (pAGO4::FLAG-AGO4-D742A) expressed in the ago4-4/ago6-2/ago9-1 (CS72560) homozygous triple mutant background. Mutagenesis of the catalytic motif of AGO4 was performed by overlapping extension PCR. Hygromycin resistant. Homozygous for the transgene.
Growth Requirement: none
Marker: BASTA, hygromycin, kanamycin
Background: Col (Columbia); Ws (Wassilewskija)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
partial complementation of small RNA accumulation
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1111/tpj.13463 | 28002617 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pGKB5 | promoter trap T-DNA | GUS | BASTA, kanamycin | |
| pROK2 | T-DNA | kanamycin | ||
| pAGO4::FLAG-AGO4-D742A | slicing-defective AGO4 driven by the native AGO4 promoter | AGO4 | hygromycin |
Quality Control Comments
There is no quality control data for this stock.