NASC stock number: N72601
donor stock number: TG20_pMDC139:I:35S:MDAR4Yh:GUS
Resource Type: seed
Availability: available
Donors:- Dessiree Zerpa Catanho
- Ray Ming
Donation Date: 08/03/2021
Date Released: 09/24/2021
Description:
Transgenic line. Homozygous SALK_068667C plants transformed with the pMDC139 vector containing an isoform of the CpMDAR4-Yh allele for an experiment on the characterization of CpMDAR4 gene function. Kanamycin and hygromycin resistant. Segregating for the transgene. Donated seeds are T1 generation. Construct used to generate stock: CD3-2885 / Yhiso.
Growth Requirement: Use 0.5X MS selective medium containing 25 ug/ml Hygromycin and 1% Sucrose for germination. Keep the plates in dark conditions at 4 C for at least 4 days, then place them in a growth chamber with a 16 h light and 8 h dark photoperiod at 23 C.
Marker: kanamycin, hygromycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1007/s00497-022-00447-2 | 35920937 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pROK2 | T-DNA | kanamycin | ||
| 35S:MDAR4Yh(I):GUS | CpMDAR4-Yh isoform in the pMDC139 vector | CaMV 35S | GUS | kanamycin, hygromycin |
Quality Control Comments
There is no quality control data for this stock.