NASC stock number: N72665
donor stock number: PRR7::PRR7-LUC
Resource Type: seed
Availability: available
Donors:- David Somers
- Yeon Jeong Kim
Donation Date: 10/25/2021
Date Released: 11/02/2021
Description:
Transgenic line expressing a PRR7::PRR7-luciferase translational fusion. To generate the transgenic line, the PRR7 coding sequence was subcloned into a pPZP-BAR DONR plasmid harboring luciferase fused to 1208 bp of the PRR7 promoter. A genomic fragment containing 2223 bp upstream of the 5 end of PRR7 was then cloned upstream of the PRR7 to replace the 1208 bp promoter. Col-0 plants were transformed by the floral dip method. A selected T2 line was backcrossed to Col-0, and a homozygous F3 segregant was obtained. BASTA resistant.
Growth Requirement: none
Marker: BASTA
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.3389/fpls.2019.00667 | 31191580 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| PRR7::PRR7-LUC | PRR7::PRR7-luciferase translational fusion | PRR7 | LUC | BASTA |
Quality Control Comments
There is no quality control data for this stock.