NASC stock number: N72722
donor stock number: TIR1-mCitrine#4
other name: MP3864
Resource Type: seed
Availability: available
Donors:- Mike Prigge
- Mark Estelle
Donation Date: 11/15/2021
Date Released: 01/07/2022
Description:
Trangenic line: pTIR1:gTIR1-mCitrine in the tir1-10 (SALK_090445; CS69649) background. Genomic fragment includes 2303 bp upstream and 742 bp downstream from the coding region with a GARGGGGGA linker followed by mCitrine inserted at the native stop codon. BASTA resistant. Homozygous for the transgene.
Growth Requirement: none
Marker: BASTA, kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
less YFP signal than line 2 (CS72721) but more than TIR1-Venus (CS69661)
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.7554/eLife.54740 | 32067636 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pROK2 | T-DNA | kanamycin | ||
| pTIR1:gTIR1-mCitrine | genomic fragment includes 2303 bp upstream and 742 bp downstream from the coding region with a GARGGGGGA linker followed by mCitrine inserted at the native stop codon | pTIR1 | mCitrine | BASTA |
Quality Control Comments
There is no quality control data for this stock.