NASC stock number: N72730
donor stock number: sgs3-13 cpFLIPPi-5.3m
Resource Type: seed
Availability: available
Donors:- Wayne Versaw
Donation Date: 11/16/2021
Date Released: 12/13/2021
Description:
cpFLIPPi-5.3m FRET-based sensor for inorganic phosphate (Pi) constitutively expressed from the UBQ10 promoter. The construct was introduced into the siRNA mutant sgs3-13 to minimize potential loss of fluorescent signals caused by posttranscriptional gene silencing. BASTA resistant. Transgene genotype unknown.
Growth Requirement: none
Marker: BASTA, kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
sensor protein localizes to cytosol
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1104/pp.114.254003 | 25624397 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pROK2 | T-DNA | kanamycin | ||
| cpFLIPPi-5.3m | inorganic phosphate FRET-based sensor expressed from the UBQ10 promoter | UBQ10 | CFP;cpVenus | BASTA |
Quality Control Comments
There is no quality control data for this stock.