NASC stock number: N72739
donor stock number: PGX2 OE
Resource Type: seed
Availability: available
Donors:- Avry Mudersbach
- Charles Anderson
Donation Date: 11/17/2021
Date Released: 01/14/2022
Description:
Transgenic line expressing PGX2 under the control of the strong cauliflower mosaic virus 35S promoter. A full-length synthetic PGX2 coding sequence was cloned into pEarleyGate 104 using the Gateway cloning system to generate a 35S:YFP-PGX2 construct. Col plants were transformed using the floral dip method. Positive transformants were screened on MS plates containing 5 um MSO. Homozygous for the transgene.
Growth Requirement: none
Marker: methionine sulfoximine
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
enhanced hypocotyl elongation
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1111/tpj.13453 | 28004869 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| 35S:YFP-PGX2 | PGX2 overexpression construct | CaMV 35S | YFP | methionine sulfoximine |
Quality Control Comments
There is no quality control data for this stock.