NASC stock number: N72743
donor stock number: PLL12-comp
Resource Type: seed
Availability: available
Donors:- Avry Mudersbach
- Charles Anderson
Donation Date: 11/17/2021
Date Released: 01/14/2022
Description:
PLL12 complementation line. The 2 kb PLL12 native promoter and PLL12 coding sequence were ligated and cloned into the pCR8/GW/TOPO entry vector, then LR cloned into vector pMDC110. The plasmid was transformed into pll12-1 (SAIL_1207_A07/CS878465) heterozygous plants and selected using methionine sulfoximine and hygromycin to obtain homozygous lines for both alleles.
Growth Requirement: none
Marker: BASTA, hygromycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1093/plcell/koab161 | 34109391 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pDAP101 | T-DNA | BASTA | ||
| PLL12pro:PLL12 | PLL12 complementation construct | PLL12pro | hygromycin |
Quality Control Comments
There is no quality control data for this stock.