NASC stock number: N72754
donor stock number: pHAM2::2xYPET-N7mirS in Ler
Resource Type: seed
Availability: available
Donors:- Elliot Meyerowitz
Donation Date: 11/29/2021
Date Released: 02/04/2022
Description:
Transgenic microRNA-sensitive fluorescence reporter line for HAM2. The 3,122 bp HAM2 promoter was amplified and cloned in front of 2xYPET-N7mirS; and the 1,149 bp HAM2 3’ untranslated sequence was PCR amplified and cloned 3’ of the 2xYPET-N7mirS construct. The fused DNA fragment was then cloned into pMOA34 and Ler plants were transformed. Hygromycin selection. Homozygous for the transgene.
Growth Requirement: hygromycin selection
Marker: hygromycin
Background: Ler (Landsberg erecta)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1038/nature13853 | 25363783 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pHAM2::2xYPET-N7mirS | microRNA-sensitive fluorescence reporter construct | pHAM2 | 2xYPET | hygromycin |
Quality Control Comments
There is no quality control data for this stock.