NASC stock number: N73470
donor stock number: GFP-FIE amiRNA #1
Resource Type: seed
Availability: available
Donors:- Jen Sheen
Donation Date: 06/29/2023
Date Released: 06/30/2023
Description:
The pFIE:Flag-GFP-FIE construct was introduced into PLB21 amiRNA fie #2 by genetic crossing, antibiotic selected, and confirmed by PCR-based genotyping and immunoblot analyses. 10 mM oestradiol was added into the medium at the beginning of germination to induce FIE depletion. Without oestradiol, plant showed normal development.
Growth Requirement: none
Marker: kanamycin, hygromycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
grossly aberrant developmental phenotypes upon induction with oestradiol, including early flowering, abnormalities in the size, morphology and patterning of leaves, flowers and siliques, reduced branching with miniature plant architecture and terminal flowers and infertility
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1038/s41586-022-05171-5 | 36104568 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pFIE:Flag-GFP-FIE | Includes 2.5 kb upstream and 1.3 kb downstream sequences of the FIE coding region in the pCAMBIA1300 binary vector. The triple Flag-tag sequence was introduced along with the promoter sequence and GFP was inserted between the Flag sequence and the first ATG codon sequences. | GFP | hygromycin | |
| fie-amiR-es | FIE amiRNA was amplified and transferred to the oestradiol-inducible vector pLB12 | kanamycin |
Quality Control Comments
There is no quality control data for this stock.