NASC stock number: N73562
donor stock number: pLH13 in pML1::KRP1 as2-7D
Resource Type: seed
Availability: available
Donors:- Adrienne Roeder
Donation Date: 03/25/2024
Date Released: 04/03/2024
Description:
Transgenic line expressing pML::KRP1 and fluorescent membrane marker pLH13 (p35S::mCitrine-RCI2A) in an as2-7D mutant background. The as2-7D allele has a G to A mutation at base 1,484 upstream of the ATG translational start site of AT1G65620. pML::KRP1 consists of Kip-related protein 1 (KRP1) under the control of the MERISTEM LAYER 1 (ML1) promoter. pLH13 consists of mCitrine tagged to RARE-COLD-INDUCIBLE 2A (RCI2A) protein driven by the CaMV 35S promoter. The membrane marker silences frequently, and hence requires screening in each generation.
Growth Requirement: none
Marker:
Background:
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
The sepals exhibit a strong rescue of the folds and outgrowths, phenotype observed on the outer epidermis of as2-7D sepals. Similar to ML1::KRP1 Ler-0 also exhibits a significant increase in the number and size of giant cells as compared to as2-7D. The sepals fail to enclose the developing flowers. The plants have an overall short stature than ML1::KRP1 Ler-0.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1101/2023.07.22.549953 | 37546730 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| p35S::mCitrine-RCI2A | yellow flourescent protein based membrane marker (pLH13, mCitrine tagged to RARE-COLD-INDUCIBLE 2A (RCI2A) protein) driven by CaMV 35S promoter | |||
| pML1::KRP1 | Kip-related protein 1 (KRP1) under MERISTEM LAYER 1 (ML1) promoter |
Quality Control Comments
There is no quality control data for this stock.