NASC stock number: N73708
donor stock number: L1T11 L1B39 T2P2
Resource Type: seed
Availability: available
Donors:- Cassandra Maranas
- Jennifer Nemhauser
Donation Date: 06/25/2024
Date Released: 06/27/2024
Description:
Transgenic line expressing integrase-based, history-dependent recorder of gene expression, Bxb1 then PhiC31 history dependent target with p35S, with Bxb1 expression under control of the MUTE promoter. T2 seeds which are not segregated for the pMUTE::Bxb1 construct, most seedlings should show a switch from BFP to RFP expression only in the guard cells of the stomata as the integrase switch occurs near the end of the stomatal differentiation lineage.
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1101/2024.06.04.597320 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| Bxb1 then PhiC31 history dependent target with p35S | tUBQ1-NeonGreen-attPPhiC31-p35S-attPBxb1-ERtag-mtagBFP2-tUBQ10-tRBCS-NLS-mCherry-attBPhiC31-attBBxb1 | |||
| pMUTE::Bxb1 | pMUTE-Bxb1-tUBQ10 |
Quality Control Comments
There is no quality control data for this stock.